For general cultivation of less fastidious microorganisms, can be enriched with blood or other biological fluids. NUTRIENT BROTH a general purpose medium used for cultivating a wide variety of microorganisms. This dehydrated medium is irradiated with dosage **25 kGy – 68kGy which is sufficient and adequate to eliminate sterility assurance level (SAL) of 10-6(4-6). Whereas, a minimum dose of 25 kGy is desired for microbial control or sterilization4. Peptic digest of animal tissue, Beef extract and Yeast extract provide the necessary nitrogen compounds, carbon, vitamins and also some trace ingredients necessary for the growth of bacteria. Sodium chloride maintains the osmotic equilibrium of the medium. It can be used for the cultivation and enumeration of bacteria which are not particularly fastidious. Addition of different biological fluids such as horse or sheep blood, serum, egg yolk etc. makes it suitable for the cultivation of related fastidious organisms.