SKU: sku-1729
Description: 
For selective enumeration of presumptive Escherichia coli and other coliforms from milk products.     MUG LAURYL SULPHATE BROTH, MODIFIED (as per ISO)is usedfor the selectiveand enumeration and  presumptive  enumeration  of Escherichia  coli. Sodium Lauryl    sulphate    broth    promotes    a    rich  growth    and    copious    gas    production    from  small  inocula  of coliform  organisms.  Aerobic  sporing bacteria  are  completely  inhibited.Tryptoneprovides  the  nitrogen,  carbon  compounds,  vitamins  and amino acids. The addition of MUG to the medium allows the positive discrimination of E. colistrains. As themajority of E. coliproduces the β-glucuronidase enzyme, they are able to hydrolyse MUG, releasing a fluorogenic  compound.  Tryptophan  acts  as  a  substrate  for  the  indole  test.Lactose  is  the fermentable sugar. Sodium chloride maintains the osmotic balance of the medium.Dipotassium phosphateand Mono potassium   phosphate controls   the   pH   during   fermentation   of   Lactose.   Lactose-positive   bacteria metabolize  lactose  with  gas  formationis  indicated  by  using  inverted  Durham  tubes.  Incubate  at  the wished temperature for 24 hours. In case of gas formation the Durham tubes rise or/and show bubbles. Turbidity of the medium accompanied by formation of gas within 48 hours is a positive presumptive test for the presence of E. coli and/or other coliform organisms. Prepared Broth becomescloudy if stored at 2-8°C, but it should get cleared at room temperature. If there is fermentation in the tube incubated at 44°C after  8  to  24  hours,  perform  indole  test  by  adding  Kovac‘s  reagent  (TR  008).  A  positive  indole  test in  a broth  tube  showing  gas  production  at  44°C  indicates  the  presence  of Escherichia  coli. If  no  fermentation occurs  in  the  tube  incubated  at  30°C  after  24  hours,  the  primary  fermentation  is  assumed  to  be  due  to organisms other than coliforms.
Product Code: 
TM 1572