MIDDLEBROOK OADC GROWTH SUPPLEMENT

Warm up the refrigerated supplement to room temperature. Shake well to form uniform suspension. Aseptically add 1
vial to 450 ml of sterile, molten, cooled (45-50?C) TM 215 – MIDDLEBROOK 7H11 AGAR BASE W/O MALACHITE GREEN /
TM 786 – MIDDLEBROOK 7H11 AGAR BASE / TM 618 – MIDDLEBROOK 7H10 AGAR BASE, ?TBL? / TM 214 – MIDDLEBROOK
7H10 AGAR BASE / TM 590 – MIDDLEBROOK 7H9 AGAR BASE. Mix well and pour into sterile screw capped tubes or
containers.
Note: Shake well before use. ?Presence of Bovine albumin Fraction V, in the supplement may give it an opaque and
sometimes cloudy appearance. It does not interfere with the biological efficacy of the product?.