SKU: sku-1809
For the selective isolation and enumeration of Staphylococci.    MANNITOL SALT AGAR is used for the selective isolation and enumeration of Staphylococci species. It conforms  to  harmonized  USP/EP/JP  requirements.1,2,3  Chapman  formulated  Mannitol  Salt  Agar  to isolate staphylococci by inhibiting growth of most other bacteria with a high salt concentration.4Mannitol is  the  fermentable  carbohydrate  which  leads  to  acid  production,  detected  by  Phenol  red  indicator. Staphylococcus aureus ferment  mannitol  and  produce  yellow  coloured  colonies  surrounded  by  yellow zones.  The  lipase  activity  can  be  visualized  as  yellow  opaque  zones  around  the  colonies.5  Coagulase-negative strains of Staphylococcus aureus are usually mannitol non-fermenters and therefore produce pink to  red  colonies  surrounded  by  red-purple  zones.  Presumptive  coagulase-positive  yellow  colonies  of Staphylococcus  aureus   should   be   confirmed   by   performing   the   coagulase   test.   Lipase   activity   of Staphylococcus aureus can  be  detected  by  supplementing  the  medium  with  egg  yolk  emulsion.  Sodium chloride in high concentration serves as an inhibitory agent against bacteria other than Staphylococci. Beef extract  gives  essential  growth  factors  and  trace  nutrients  to  the  growing  bacteria.  Phenol  Red  is  the pH indicator.  Agar  acts  as  the  solidifying  agent.  Inoculate  Mannitol  Salt  Agar  (TMH  114)  medium  with  a small number (not more than 100 cfu) of the appropriate microorganism and incubate at 35 - 37°C for 18 - 48  hours.  Recovery  rate  is  considered  as  100%  by  sub  culturing  on  Soyabean  Casein  Digest  Agar  (TMH 103) at 35±2°C for 18 - 48 hours.
Product Code: 
TMH 114