SKU: sku-1780
For the detection enumeration of coliform bacteria.     MacCONKEY  BROTH  is  used for  the  selective  enrichment  and  enumeration  of  coliform  bacteria.  It conforms to harmonized USP/EP/JP requirements.1,2,3 MacConkey Broth is a modification of the original Bile  salt  broth  recommended  by  MacConkey,  containing  0.5%  sodium  taurocholate  and  litmus  as  an indicator.4MacConkey suggested further variations of this formula using neutral red indicator instead of litmus.5,6Childs  and  Allen  demonstrated  the  inhibitory  effect  of  neutral  red  and  substituted  the  less inhibitory Bromcresol purple.7 Pancreatic digest of Gelatin provides necessary nitrogen source. Lactose is the  carbohydrate  for  Gram-negative  lactose-fermenting  bacilli.  Sodium  chloride  maintains  the  osmotic balance of the cells. Dehydrated Ox bile inhibit the growth of Gram-positive organisms. Gram-negative bacteria  usually  grow  well  on  this  media  and  are  differentiated  by  their  ability  to  ferment  lactose. Lactose-fermenting  organisms  grow  very  well  in  MacConkey  Broth  and  produce  acid,  causing  the medium  to  turn  yellow.  Gas  is  also  produced,  which collects  in  the  Durham  tubes.  Non-fermenting organisms  produce  good  growth  but  will  not  produce acid  or  gas.  Inoculate  10ml.  MacConkey  Broth (TMH 109) medium with a small number (not more than 100 cfu) of the appropriate microorganism and incubate at 35±2°C for 18-24 hours. Subculture on a plate of MacConkey Agar (TMH 110) and incubate at 35±2°C for 18-24 hours.
Product Code: 
TMH 109