M-ENTEROCOCCUS AGAR BASE

This medium was devised by Slanetz, Bent and Bartley for the enumeration of Enterococci by the membrane filter technique. Slanetz and Bartley modified it by the addition of Triphenyl Tetrazolium Chloride (TTC) and found that larger colonies and higher counts were obtained by placing membrane filters directly on the agar surface than on pads saturated with liquid medium. This medium is highly selective. Burkwell and Hartman used polysorbate 80 (0.5 ml/l) and sodium carbonate (2 ml of a 10% aqueous solution per litre) to increase sensitivity for direct plating of foods and increasing colony size. As per standard methods, M-Enterococcus Agar is used for the detection of faecal Streptococcus and Enterococcus groups using the membrane filtration technique.

for isolation and enumeration of Enterococci in sewage, water and foods by membrane ?lter technique.