For cultivation and maintenance of recombinant strains of E.coli
LAURIA AGAR BASE is used for cultivation and maintenance of recombinant strains of E.coli. The media is nutritionally rich for the growth of pure cultures of recombinant strains. Strains derived from Escherichia coli K12 are deficient in Vitamin B synthesis are further modified by specific mutation to create auxotrophic strains and are therefore unable to grow on nutritionally deficient media. Luria Agar Base is slightly different with double amount of sodium chloride. Sodium chloride provides sodium ions for membrane transport and also maintains the osmotic equilibrium of the medium. Casein enzymatic hydrolysate provides peptides and peptones while Vitamin B complex is provided by yeast extract.