HARTLEY’S DIGEST BROTH

Hartley described the value of tryptic digest of muscle for the production of diphtheria toxin. Since then it is used as a general-purpose broth, capable of initiating the growth of demanding (fastidious) organisms from a small inocula. Hartley’s Digest Broth can be used for the recovery of fastidious organisms such as Group A, C, G Streptococci and Streptococcus pneumonia from small inocula. Douglas used the same medium to recover Corynebacterium diphtheriae, while Monckton used it in an enrichment medium for C. diphtheriae. Hartley’s Digest Broth may be used for cultivation of blood samples, sterility testing, production of diphtheria toxin etc.

for cultivation of various bacteria from blood especially Streptococci and Corynebacterium diphtheriae