SKU: sku-151
Intended Use: 
For sterility testing of biologicals and for cultivation of aerobic, anaerobic and microaerophilic organisms.     FLUID  THIOGLYCOLLATE  MEDIUMis  used  for  sterility  testing  of  biological  and  for  cultivation  of anaerobic, and microaerophilic organisms.This dehydrated medium is irradiated with dosage **25 kGy – 68kGy which is sufficient and adequate to eliminate sterility assurance level (SAL) of 10-6(5-7). Whereas, a minimum  dose  of  25  kGy  is  desired  for  microbial  control  or  sterilization7.  The  media  is  also  used  for sterility testing of antibiotics, biologicals and foods and also for determining the phenol coefficient and sporicidal  effect  of  disinfectants.  However,  it  is intended  for  the  examination  of  clear  liquid  or  water-soluble materials. Fluid Thioglycollate Medium is also routinely used to check the sterility of stored blood in  blood  banks.  The  medium  contains  Pancreatic  digest  of  casein  and  Yeast  extract  those  provides  the nitrogen and essential growth enhancers necessary for bacterial multiplication. Sodium thioglycollate and L- Cystineacts as a reducing  agent which maintains  a  low oxygen tension  by  removing  molecular  oxygen  from  the  environment. Indicator Resazurin dispensed throughout the media causing the media to appear brown or pink in colour.  Dextrose is a fermentable energy source. Addition of small amount of agar  helps  in  maintaining  low  redox  potential  for  stabilizing  the  medium.  Addition  of  agar  in  small amount  of  agar  favors  the  growth  of  aerobes  as  well  as  anaerobes  which  retards  dispersion  of  CO2, diffusion of oxygen, and reducing substances. On incubation period of 30 - 350C for 48- 72 hours shown luxuriant growth in all the culture strains tested. Sodium chloride helps maintaining the osmotic balance.
Product Code: 
TSM 318