CRAIG’S MEDIUM

Vibrio cholerae is a gram-negative bacterium that causes cholera in humans. Cholera is the prototype of diarrheal syndromes in which the disease is caused through the production of toxins that interrupt normal intra-intestinal exchange of water and electrolytes. A variety of laboratory diagnosis of cholera and toxin detection have been developed. Craigs Medium has similar composition to that of CAYE Broth, which was formulated as per APHA for cultivating V.cholerae while testing their enteropathogenicity.
For testing the enterotoxin production of V.cholerae, the culture is to be grown in Craigs Medium as this medium enhances the production of Vibrio enterotoxin. Inoculate test cultures from TN Agar slants to tubes of Craigs Medium and incubate overnight at 30 ? 2?C which is then further used for immunological testing of enterotoxigenicity.

for cultivation of Vibrio cholerae to determine its enterotoxigenicity