For isolation and differentiation of microorganisms based on lactose fermentation. C.L.E.D AGAR WITH ANDRADE INDICATOR is used for culturing of pathogens causing urinaryinfections. It improves colony detection and microorganism identification or it enhances the appearance of the colony and aids in the identification of microorganisms by the addition of the Andrade indicator. Andrade indicator is acid fuchsin in 1N sodium hydroxide. It is based on Lactose fermentation in whichend product is lactic acid which lowers the pH of the medium. Mixed culture is predominant in urine sample and test such as coagulase, oxidase, catalase and agglutination with specific sera, can be performed on primary isolates from the medium. Beef extract, Peptone and Tryptone provide the nitrogen, vitamins and amino acids in CLED Agar. L-Cystine is added as a growth supplement for Cystine–dependent coliforms. Lactose acts as a fermentable sugar. Organisms capable of fermenting lactose will lower the pH and change the color of the medium from green to yellow. Bromo thymol blue is used as a pH indicator. Agar is used as a solidifying agent.Inoculate and incubate at 35 ± 2°C for no longer than 24 hours, if lactose-fermenters predominate, the whole medium may turn pink, masking the presence of non-lactose fermenters.