For the isolation and identification of Cronobacter sakazakii from milk and milk products. The composition and performance of this media are as per specified in ISO /TS 22964: 2006.
Enterobacter species are widely distributed in nature occurring in fresh water, soil, sewage, plants, vegetables, animal and human feaces. Cronobacter sakazakii formerly known Enterobacter sakazakii has been closely associated with neonatal meningitis and sepsis (1). Chromogenic Enterobacter sakazakii Agar, Modified is recommended by ISO Committee for the isolation and identification of C.sakazakii (2). The chromogenic substrate (5-Bromo-4-chloro-3-indolyl α-D-glucopyranoside) is cleaved specifically by C. sakazakii resulting in the formation of blue green colonies. Other organisms, which do not cleave this substrate, produce colourless to slightly violet coloured colonies. Casein enzymic hydrolysate and yeast extract provides nitrogenous and carbonaceous compounds, long chain amino acids, vitamins and other essential growth nutrients. Yeast extract is source of vitamins, particularly the B-group essential for bacterial growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Sodium desoxycholate and Crystal violet inhibit gram-positive bacteria. 5-Bromo-4- chloro-3-indolyl α-D-glucopyranoside is the chromogenic substrate.