for detection and enumeration of Escherichia coli without further confirmation on membrane filter or by indole in food microbiology test. for easy enumeration of E.coli without membrane filtration, or pre-incubation on Mineral Modified Glutamate Medium Base.
The chromogenic agent X- Glucuronide used in this medium helps to detect glucuronidase activity. Escherichia coli cells absorb x-glucuronide and the intracellular glucuronidase splits the bond between the chromophore and the glucuronide. The released chromophore gives coloration to the colonies. The peptone provides the essential growth nutrients to the organisms. Bile salts mixture inhibits gram-positive organisms.