CARBOHYDRATE CONSUMPTION BROTH BASE

Carbohydrate Consumption Broth is used for the cultivation and differentiation of Listeria species and formulated as per Atlas. It is also recommended by FDA and ISO with a slight difference in the concentration of bromocresol purple. Differentiation is based on fermentation of glucose, xylose, rhamnose, ribose, a-methyl-D-mannoside and mannitol. Carbohydrate utilization test: Inoculate each kind of carbohydrate fermentation broth with one loopful of inoculum. Incubate for 7 days at 37?C. Observe daily for acid induced colour change and gas formation. Sometimes weak positive reactions may occur after 48 hours of incubation.

for cultivation and di?erentiation of Listeria species