CAMPYLOBACTER SUPPLEMENT-II (Butzler)

Rehydrate the contents of one vial aseptically with 5 ml of 50% aqueous ethanol and mix well to dissolve. Avoid frothing
of the solution. Aseptically add the contents to 500 ml of sterile, molten, cooled (45-50?C) TM 071 – COLUMBIA BLOOD
AGAR BASE / TMV 071 – COLUMBIA BLOOD AGAR BASE (VEG.) / TM 2044 – COLUMBIA BLOOD AGAR BASE W/ 1% AGAR /
TM 041 – BLOOD AGAR BASE No. 2 / TM 1842 – BLOOD AGAR BASE NO. 2 (ISO 11290-1:2017) / TM 2011 – BLOOD AGAR
BASE NO. 2 WITH 1.2% AGAR / TM 514 – BRUCELLA AGAR BASE / TMV 514 – BRUCELLA AGAR BASE (VEG.) along with 5%
v/v defibrinated Sheep blood or 5-7% v/v laked horse blood and rehydrated contents of 1 vial of Camplyobacter Growth
Supplement. Mix gently and pour into sterile petri plates.