For isolation of Clostridium botulinum from faecal samples associated with food borne & infant botulism. C. BOTULINUM ISOLATION AGAR BASEis used forisolation of Clostridium botulinum from faecal samples associated with food borne & infant botulism.Clostridium botulinumis anaerobic, Gram positive, spore forming group of bacteria and are responsible forthe food borne disease called botulism. The organism produces a neurotoxin –botulin which causes the disease botulism. Botulinal toxin is heat-labile. Therefore the test samples and cultures should be maintained at refrigeration temperatures.The flaccidmuscular paralysis occurs due to the effect of the toxin. Medium contains Casein enzymatic hydrolysate and Yeast extractare added to provide the carbon, nitrogen and vitamins for the growth of the organisms in the medium.Dextrose as source of carbohydrate and energy.Sodium chloride (to maintain osmotic balance)and Disodium phosphate (buffering agent). Magnesium sulphate helps in cell division, growth and enhancessporulation of microorganisms. Agar is used as a solidifying agent. Supplement Egg Yolk Emulsion helps in detecting lecithinase, lipase and proteolytic activity.The antibiotic supplementcontainsbroad spectrum antibiotics;Cycloserine, Sulphamethoxazole and Trimethoprim makes the medium very selective.