SKU: sku-955
Intended Use: 
For pre enrichment of injured Salmonella species prior to selective enrichment and isolation.    BUFFERED PEPTONE WATER(VEG.) is used for pre enrichment of injured Salmonella species prior to selective enrichment and isolation and to repair damaged cell of Salmonellae species before transfer to a selective  medium  by  maintaining  a  high  pH  over  the pre-enrichment  period.  The  high  pH  capacity  is especially  useful  for  vegetable  samples  which  have a  low  buffering  capacity.  The  media  contains  Veg. Peptone  as  a  source  of  carbon,  nitrogen,  vitamins  and  minerals.  Sodium  chloride  maintains  the  osmotic balance and phosphates buffer the medium. The broth is rich in nutrients and produces high resuscitation rates for sublethally injured bacteria and supports intense growth. The phosphate buffer system prevents bacterial  damage  due  to  changes  in  the  pH  of  the  medium.  This  pre-enrichment  medium  is  free  from inhibitors and is well buffered and provides conditions for resuscitation of the cells that have been injured by  processes  of  food  preservation.  This  medium  can be  used  for  testing  dry  poultry  feed.  In  a  survey involving  isolation  of Salmonellae  from  meat  that  had  been  artificially  contaminated with  sub-lethally injured organisms, pre-enrichment in Buffered Peptone Water (Veg.) at 37°C for 18 hours before selection in Tetrathionate Brilliant Green Bile Broth (TM 442) showed superior results compared with direct selection method. Lactose Broth (TM 1214) is frequently used as a pre-enrichment medium but it may be detrimental to recovery of Salmonellae. Inoculate 10 grams specimen in 50 ml of these media and incubate at 35 - 37°C for 18 hours. Transfer 10 ml from this medium to 100 ml of Tetrathionate Broth (TM 413) and incubate at 43°C for 24 - 48 hours and then subculture on selective plating media.
Product Code: 
TMV 307