For pre-enrichment of injured Salmonella species prior to selective enrichment and isolation. It is recommended by BIS committee under specification IS:5887 (Part III) 1999, reaffirmed 2005. BUFFERED PEPTONE WATERis used for pre enrichment of injured Salmonellaspecies prior to selective enrichment and isolationand to repair damaged cell of Salmonellae species before transfer to a selective medium by maintaining a high pH over the pre-enrichment period. The high pH capacity is especially useful for vegetable samples which have a low buffering capacity.The media containsPeptone as a source of carbon, nitrogen, vitamins and minerals. Sodium chloride maintains the osmotic balance andphosphates buffer the medium. The broth is rich in nutrients and produces high resuscitation rates for sublethally injured bacteria and supports intense growth. The phosphate buffer system prevents bacterial damage due to changes in the pH of the medium. This pre-enrichment medium is free from inhibitors and is well buffered and provides conditions for resuscitation of the cells that have been injured by processes of food preservation. This medium can be used for testing dry poultry feed. In a survey involving isolation of Salmonellaefrom meat that had been artificially contaminated with sub-lethally injured organisms, pre-enrichment in Buffered Peptone Water at 37°C for 18 hours before selection in Tetrathionate Brilliant Green Bile Broth (TM 442) showed superior results compared with direct selection method. Lactose Broth (TM 1214) is frequently used as a pre-enrichment medium but it may be detrimental to recovery of Salmonellae. Inoculate 10 grams specimen in 50 ml of these media and incubate at 35-37°C for 18 hours. Transfer 10 ml from this medium to 100 ml of Tetrathionate Broth (TM 413) and incubate at 43°C for 24 -48 hours and then subculture on selective plating media.