For detection and confirmation of coliform bacteria in water and foods. BRILLIANT GREEN BILE BROTH 2% (BRILLIANT GREEN LACTOSE BILE BROTH 2%) is used for the detection of coliform organisms in foods, dairy products, water and wastewater, as well as in other materials of sanitary importance. Brilliant Green Bile Broth 2% contains two inhibitors of both gram-positive and selected gram-negative organisms;i.e., Oxgall and Brilliant green dye. Peptic digest of animal tissueprovides the essential nutrients for growth: nitrogen, vitamins, minerals and amino acids. Organisms, primarily coliforms, which are resistant to the action of the inhibitors and which ferment Lactose are able to replicate in this medium. Fermentation is detected by gas production. Gas production within 48 hours is considered positive evidence of fermentation by coliforms.