For detection and confirmation of coliform bacteria in water and foods. BRILLIANT GREEN BILE BROTH 2%(VEG) isused for detection and confirmation of coliform bacteria in water and foods. It is one of the most widely used medium for this purpose. This medium isthe modification of Brilliant green bile broth 2% which is usedfor the presumptive identification and confirmation of coliform bacteria. Lactose is the fermentable carbohydrate. Gram positivebacteria are inhibited by brilliant greenand synthetic detergent. Production of gas from lactose fermentation is detected by incorporating inverted Durhams tube, which indicates the positive evidence of faecal coliform since non faecal coliforms growing in this mediumdo not produce gas. During examinations of water sample, growth from presumptive positive tubes showing gas in Lactose Broth (TM 757) or Lauryl Tryptose Broth (TM 150) is inoculated in Brilliant Green broth 2% (TMV 365). Gas formation within 48±2hours confirms the presumptive test.