SKU: sku-911
Intended Use: 
For enumeration of coliform bacteria in water and foods.     BRILLIANT  GREEN  BILE  AGAR is  used for  enumeration  of  coliform  bacteria  in  water  and  foods. It uses Basic fuchsin  to  differentiate  between  positive  lactose-fermenting  and  lactose-non-fermenting bacteria.The Peptic  digest  of  animal  tissueprovides  the  essential  nutrients  for  growth:  nitrogen, vitamins,  minerals  and  amino  acids.Lactose  is  the  fermentable  carbohydrate  providing  carbon  and energy.Ox  bile  and  Brilliant  green  inhibit  Gram-positive  bacteria  and  most  Gram-negative  bacteria except Coliforms.Acetaldehyde  production  by  lactose-fermenting  organisms,  such  as E.coli, produce characteristic red colonies with a pink surrounding area, marked by its reaction with Sodium sulphite in the presence of Basic fuchsin, a pH indicator. Lactose non-fermenters form colorless, transparent colonies.For the enumeration of coliform bacteria employ sample dilutions, which yield between 10 -50 colonies per  plate  using  the  pour  plate  method.Therefore,  several  dilutions  should  be  made  in  the  melted medium, poured and once gelled, incubated at 35 ± 2°C for 18 -24 hours. The coliform colonies have an intensely  red  center  zone  surrounded  by  a  pink  halo  sharply  outlined  against  the  uniformly  blue background of the medium. Salmonella spp.which do not ferment Lactose, produce colorless to pale pink colonies.The  medium  is  sensitive  to  light,  which  reduces  its  effectiveness  and  changes  its  color  from strong blue to purple or pink. The medium shouldbe prepared immediately before use and, if necessary, stored in the dark for as little time as possible
Product Code: 
TM 048

Product Category: