For testing the sterility of biological products and for cultivation of enteric, anaerobic and microaerophilic organisms. These media are prepared by completely replacing animal based peptones with vegetable peptones. Brewer Thioglycollate Veg medium is the modification of Brewer Thioglycollate Medium prepared as per the original formula of Brewer (1, 2). Brewer Thioglycollate Veg Medium Modified is a modification of Linden Thioglycollate Medium (3). This medium contains highly nutritious Veg peptone No.3, Veg infusion and Veg hydrolysate which support luxuriant growth of even fastidious bacteria. Sodium thioglycollate helps to create anaerobic condition as well as neutralizes toxicity of mercurial compounds if present in the inoculum of the test material. Very small amount of agar present maintains anaerobic conditions at the bottom of the broth. Methylene blue is an oxidation-reduction indicator, indicating oxygen content of the medium by exhibiting bluish-green colour to the medium in presence of oxygen. The uninoculated medium shows bluish green colour at the top indicating presence of oxygen in that part. Modified medium contains more thioglycollate and was recommended for sterility testing procedures. Organisms which ferment dextrose and lower the pH to critical levels may not survive in this medium after growth has taken place.