for cultivation of fastidious microorganisms associated with blood culture. BHI Medium is useful for cultivating a wide variety of microorganisms since it is a highly nutritive medium. This medium is recommended by Food and Drug Administration. This culture medium complies with the specifications given by EN ISO 6888 and APHA. It is also used to prepare the inocula for antimicrobial susceptibility testing. BHI Broth is a modification of the original formulation of Rosenow, where he added pieces of brain tissues to dextrose broth. BHI Broth is also the preferred medium for anaerobic bacteria, yeasts and moulds. This medium is nutritious and well buffered to support the growth of wide variety of organisms. With the addition of 10% defibrinated sheep blood, it is useful for isolation and cultivation of Histoplasma capsulatum and other fungi. For selective isolation of fungi, addition of gentamicin and/or chloramphenicol is recommended. This medium is not suitable for obtaining characteristic haemolytic reactions even after addition of blood because of its glucose content.
The nutritionally rich base of Beef heart and Calf brain infusions and Peptone mixture provide nitrogen, vitamins, minerals and amino acids essential for growth of a variety of microorganisms. Dextrose is the carbon energy source and Sodium chloride maintains the osmotic balance. Disodium phosphate helps in maintaining the buffering action of the medium. This medium is very versatile and supports the growth of many fastidious organisms. With the addition of 0.1% agar, the medium is used for the cultivation of anaerobes. Adding 0.1% agar reduces the flow of oxygen convection currents and encourages the development of anaerobes and microorganisms.