SKU: sku-148
For cultivation of fastidious organisms associated with blood culture.    BRAIN  HEART  INFUSION  BROTH   is   used   for   cultivation   of   fastidious   and   non   fastidious microorganisms,  including  aerobic  and  anaerobic  bacteria.  This  dehydrated  medium  is  irradiated  with dosage **25 kGy – 68kGy which is sufficient and adequate to eliminate sterility assurance level (SAL) of 10-6(3-5).  Whereas,  a  minimum  dose  of  25  kGy  is  desired  for microbial  control  or  sterilization3.  Scientists ‘Rosenow’  with  other  investigators  confirmed  the  media  supports  growth  of Streptococci, Pneumococci,Meningococci, Histoplasma  capsulatu  etc.  It  permits  the  cultivation  of  gonococci  on  addition  of  10% defibrinated  sheep  blood.  BHI  broth  is  also  used  for  preparing  the  inoculum  used  in  antimicrobial susceptibility tests.The mixture of Peptone, Brain and heart infusions provides organic nitrogen, carbon, and  vitamins.  The  mixture  of  infusions is  particularly  useful  in  the  isolation  of Actinomyces israeli  and Histoplasma capsulatum. Dextrose is  the carbohydrate source.   A low  concentration  of  dextrose  is used to stimulate early growth. Sodium chloride maintains the osmotic environment. Disodium phosphate is the buffering agent in this medium. With the addition of 7% defibrinated blood the medium will support thegrowth  of  a  wide  range  of  fastidious  and  nonfastidious  organisms,  the  phosphate  buffer  will  help neutralise  the  acids  produced  from  the  utilisation of  dextrose  and  thus  maintain  viability.  The  culture should  be  examined  in  a  closed  filtered  air  cabinet,  due  to  its  highly  infective  spores.  The  medium  islightly  buffered  to  prevent  the  early  death  of  some  species  due  to  acid  production.  Organisms  which produce  significant  amount  of  acid  may  well  overwhelm  the  buffering  system  and  auto-sterilise.  BHI Broth can be supplemented with antibiotics, varying amounts varying amounts of Sodium chloride, Yeast extract,  and  serum  to  provide  a  rich  medium  for  bacteria,  yeasts  and  pathogenic  fungi.The  addition  of 0.1%  Agar  can  be  used  to  lower  oxygen  tension,  providing  an  atmosphere  to  support  the  growth  of aerobic, microaerophilic, and obligate anaerobic microorganisms.
Product Code: 
TSM 362