For cultivation of fastidious organisms associated with blood culture. BRAIN HEART INFUSION BROTH is used for cultivation of fastidious and non fastidious microorganisms, including aerobic and anaerobic bacteria. This dehydrated medium is irradiated with dosage **25 kGy – 68kGy which is sufficient and adequate to eliminate sterility assurance level (SAL) of 10-6(3-5). Whereas, a minimum dose of 25 kGy is desired for microbial control or sterilization3. Scientists ‘Rosenow’ with other investigators confirmed the media supports growth of Streptococci, Pneumococci,Meningococci, Histoplasma capsulatu etc. It permits the cultivation of gonococci on addition of 10% defibrinated sheep blood. BHI broth is also used for preparing the inoculum used in antimicrobial susceptibility tests.The mixture of Peptone, Brain and heart infusions provides organic nitrogen, carbon, and vitamins. The mixture of infusions is particularly useful in the isolation of Actinomyces israeli and Histoplasma capsulatum. Dextrose is the carbohydrate source. A low concentration of dextrose is used to stimulate early growth. Sodium chloride maintains the osmotic environment. Disodium phosphate is the buffering agent in this medium. With the addition of 7% defibrinated blood the medium will support thegrowth of a wide range of fastidious and nonfastidious organisms, the phosphate buffer will help neutralise the acids produced from the utilisation of dextrose and thus maintain viability. The culture should be examined in a closed filtered air cabinet, due to its highly infective spores. The medium islightly buffered to prevent the early death of some species due to acid production. Organisms which produce significant amount of acid may well overwhelm the buffering system and auto-sterilise. BHI Broth can be supplemented with antibiotics, varying amounts varying amounts of Sodium chloride, Yeast extract, and serum to provide a rich medium for bacteria, yeasts and pathogenic fungi.The addition of 0.1% Agar can be used to lower oxygen tension, providing an atmosphere to support the growth of aerobic, microaerophilic, and obligate anaerobic microorganisms.