For detection of fastidious microorganisms in blood. BRAIN HEART INFUSION BROTHis used as Blood culturing system for the detection of microorganisms. Medium can also be used for the selective isolation of fastidious fungi (especially of Histoplasma capsulatumand Blastomyces), the growth of the accompanying bacterial and saprophyticyeasts and moulds flora can be almost completely suppressed by adding components mixtures such as: 12 mg Penicillin and 40μg Streptomycin per litre; 50mg Chloramphenicol and 500mg Cycloheximide per litre; 50mg Gentamicin and/or 50mg Chloramphenicol per litre. BHI is an enriched non-selective broth medium that is useful in the cultivating of fastidious and non-fastidious microorganisms. This medium will also support the growth of aerobic microorganisms from a variety of clinical and non-clinical specimens.The basal medium is infusion from brains and beef heart and supplemented with vitamin K1 and hemin as growth factors for most anaerobes. Hemolytic activity does not occured when blood has been added due to its glucose content. On handling Histoplasma capsulatumhighly care should be taken to avoid dissemination of its infective spores. The culture should be examined in a closed filtered air cabinet after the incubation period of 24hours at 35-37ºC.