for cultivation of fastidious pathogenic bacteria, yeasts and molds.
BRAIN HEART INFUSION AGAR is used for cultivation of fastidious and non fastidious microorganisms, including aerobic and anaerobic bacteria. Scientists ‘Rosenow’ with other investigators confirmed the media supports growth of Streptococci, Pneumococci, Meningococci, Histoplasma capsulatu etc. It permits the cultivation of gonococci on addition of 10% defibrinated sheep blood. The mixture of brain and heart infusions provides organic nitrogen, carbon, and vitamins. The mixture of infusions is particularly useful in the isolation of Actinomyces israeli and Histoplasma capsulatum. Dextrose is the carbohydrate source. A low concentration of dextrose is used to stimulate early growth. Sodium chloride maintains the osmotic environment. Disodium phosphate is the buffering agent in this medium. With the addition of 7% defibrinated blood the medium will support the growth of a wide range of fastidious and nonfastidious organisms, the phosphate buffer will help neutralise the acids produced from the utilisation of dextrose and thus maintain viability. The culture should be examined in a closed filtered air cabinet, due to its highly infective spores. The medium is lightly buffered to prevent the early death of some species due to acid production. Organisms which produce significant amount of acid may well overwhelm the buffering system and auto-sterilise. BHI agar can be supplemented with antiobiotics, varying amounts varying amounts of Sodium chloride, Yeast extract, and serum to provide a rich medium for bacteria, yeasts and pathogenic fungi.