For isolation and cultivation of fastidious organisms after addition of blood. BLOOD AGAR BASE W/ LOW pH medium is used for the isolation and cultivation of fastidious organisms after addition of blood. The Blood Base w/ low pH medium contain highly nutritious extract of Beef heart, which provide vitamins, salts and other organic nitrogen compounds. Sodium chloride provides essential ions and maintains electrolyte balance. Tryptone provides additional growth factors in the medium. Agar is a solidifying agent. Supplementation with blood (5%) provides additional use in isolation, cultivation, and determination of hemolytic reactions, of fastidious pathogenic microorganisms. Haemolytic patterns may vary with the source of animal blood or type of base medium used showed none of the hemolytic activity with good growth pattern of culture after incubation period of 48 hours. By using sheep blood, V factor destroying enzyme ( nucleotidase ) which prevents the growth of Hemophilus species on sheep blood agar unless another microorganisms, eg., Staphylococci, provides the V factors. Small amounts of reducing sugars inhibit the expression of β-hemolytic, and β -hemolytic Streptococci may develop a green zone or ring of hemolysis.