SKU: sku-864
For selective isolation of Salmonellae from faeces, urine, sewage and other materials.  Bismuth Sulphite Agar (Veg)  is prepared by using Veg special peptone and Veg extract which is free  of  BSE/TSE  risks.  Bismuth  Sulphite  Agar  is  the  modification  of  Wilson  and  Blair  formula, which is recommended by various Associations for the isolation and preliminary identification of Salmonella serotype Typhi and other Salmonellae  from  pathological materials, sewage,  water, food and other products.         Bismuth Sulphite Agar (Veg) is the modification of Bismuth Sulphite Agar where all animal based peptones are replaced with Veg peptones. Bismuth Sulphite Agar was stable, sensitive and found to be superior to Wilson’s original medium. Brilliant green and bismuth sulphite incorporated into the  medium  inhibit  the  intestinal  gram-negative  and  gram-positive  bacteria. Salmonella serotype Typhi, Salmonella serotype Enteritidis and Salmonella Serotype Typhimurium typically grow as black colonies with a surrounding metallic sheen resulting from hydrogen sulfide (H2S) production and reduction of sulphite to black ferric sulphide. Salmonella serotypeParatyphi A grow as light green colonies. Also, this medium favor use of larger inoculum as compared to other selective media, as it has unique inhibitory action towards gram-positive and coliform organisms. The medium may be  inhibitory  to  some  strains  of Salmonella species  and  therefore  should not  be  used  as  the  sole selective medium for these organisms. Shigella species are mostly inhibited on this medium and also  some Salmonellae like Salmonella serotypeSendai, Salmonella serotype Berta, Salmonella serotype Gallinarum are inhibited.           Colonies  on  Bismuth  Sulphite  Veg  Agar  may  be  contaminated  with  other  viable  organisms; therefore,  isolated  colonies  should  be  subcultured on  to  a  less  selective  medium.  Veg  special peptone  and  Veg  extract  provide  nitrogen,  vitamins and  minerals.  Dextrose  acts  as  an  energy source.  Ferrous  sulphate  is  used  for  detection  of  hydrogen  sulfide  (H2S)  production.  Disodium phosphate buffers the medium.
Product Code: 
TMV 039