SKU: sku-145
Intended Use: 
For selective isolation and identification of Salmonellae from faeces, urine, sewage and other materials.   BISMUTH SULPHITE AGAR is used for the selective isolation of Salmonella spp. Salmonella constitute the most taxonomically complex group of bacteria among Enterobacteriaceae. This dehydrated medium is  irradiated  with  dosage  **25  kGy  –  68kGy  which  is  sufficient  and  adequate  to  eliminate  sterility assurance level (SAL) of 10-6 (8-10). Whereas, a minimum dose of 25 kGy is desired for microbial control or sterilization8. Bismuth Sulphite Agar is a modification of Wilson and Blair formula. Also this medium favors use of larger inoculum as compared to other selective media, as it has unique inhibitory actiontowards gram-positive organisms and coliforms. Peptone and Beef extract serve as sources as carbon, nitrogen,  vitamins  and  essential  growth  factors.  Bismuth  Sulphite  Indicator  and  Brilliant  Green  are complementary, inhibiting to Gram-positive bacteria and coliforms, allowing Salmonella spp. to grow. Ferrous Sulphate is used for H2S production. When H2S is present, the iron incorporated in medium is precipitated, and positive cultures produce the characteristic brown to black color with metallic sheen. Agar is the solidifying agent.
Product Code: 
TSM 039