For detection of microorganisms in blood. BHI Supplemented w/ 0.05% is used for detection of microorganisms, including aerobic and anaerobic bacteria. Brain Heart Infusion Broth is a modification of the original formulation of Rosenow, where he added pieces of brain tissues to dextrose broth. Brain Heart Infusion Broth is also the preferred medium for anaerobic bacteria, yeasts and moulds.The media is especially suited for the cultivation of staphylococci for the plasma coagulase test and for setting up blood cultures. Addition of ascites permits the cultivation of gonococci. With the addition of 10% defibrinated sheep blood, it is useful for isolation and cultivation of Histoplasma capsulatumand other fungi. For selective isolation of fungi, addition of gentamicin and/ or chloramphenicol is recommended.BHI broth is also used for preparing the inoculum used in antimicrobial susceptibility tests. Beef heart, Brain infusions and Peptone provides organic nitrogen, carbon, and vitamins. Dextrose is carbohydrate source. Low concentration of dextrose is used to stimulate early growth. Sodium chloride maintains the osmotic environment. Disodium phosphate is buffering agent. BHI Broth can be supplemented Sodium polyanethol sulfonate(SPS)which isused as a non-toxic anticoagulant which enables bacterial growth and prevents the action of natural bacterial inhibitors of blood.