SKU: sku-822
For isolation and enumeration of coagulase positive Staphylococci from food and other products.    BAIRD  PARKER  AGAR  BASE  is  used  for  the  isolation  and  enumeration  of  coagulase  positive Staphylococci. Veg. CEH enzymatic hydrolysate, Veg. BE extract are the source of carbon and nitrogen. Yeast extract provides vitamins (B - complex) which helps in stimulating bacterial growth. The  selectivity  of  the  medium  is  maintained  by  the  addition  of  Lithium  chloride  and  3.5 %  of  Potassium  Tellurite    solution.  Both    are    helpful  in    suppressing  the    growth    of    other    organism    except  Staphylococci  sp. Lithium chloride and potassium tellurite inhibit the accompanying flora. Glycine and Sodium pyruvate stimulate the growth of Staphylococci. Staphylococci that contain lecithinase break down the egg yolk and form clear zones around the colonies. Black colonies are formed due to reduction of the Potassium tellurite to tellurium. The plates should be dry before inoculation (the drying can be done by incubating at 35 ± 2°C for approximately 10 minutes before use). Prepare the sample in an adequate solution, dilute it and place from 0.1 ml to 1.0 ml of the appropriate dilution in the plates. Spread the inoculum over the entire surface. Typical S. aureus colonies are black, shiny, and convex and surrounded by a clear zone of approximately 2 - 5   mm in diameter. Some other micro-organisms, which occasionally grow  on  this  medium,  are micrococci that  form  small  dark  or  black  colonies, yeasts that  form  white colonies and some species of Bacillus that form dark brown matte colonies.
Product Code: 
TMV 358