For cultivation of Pseudomonas aeruginosa from water by membrane filter technique. ASPARAGINE PROLINE BROTH (VEG.) is used for cultivation of Pseudomonas aeruginosa from water by membrane filter technique. This Medium contains Potassium sulphate and Phosphates salts which acts as a buffer system. Magnesium sulphate readily provides magnesium ions to the medium for various enzymatic reactions. Asparagine & Proline containing enantiomeric forms those can be readily utilized for the growth of Pseudomonas sp. P. aeruginosa hydrolyze asparagine to aspartic acid. The appearance of growth with or without fluorescent pigmentation is considered a presumptive test for the presence of P. aeruginosa and counts are determined using the MPN tubes.