ANTIBIOTIC ASSAY MEDIUM NO. 35 (VEG.)

Antibiotic Veg Assay Medium No. 35 is prepared by incorporating vegetable peptones in place of animal peptones, making the medium BSE-TSE risks free. This can be used for the same purpose of Antibiotic Assay Medium No. 35 which is formulated in accordance with CFR.
Addition of glycerol is important for provision of carbon to the test organism. To perform the antibiotic assay, the Base Agar should be prepared on the same day as the test. For the cylinder method, a base layer of 21 ml is required. Once the base medium has solidified, seed layer inoculated with the standardized culture can be overlaid. Even distribution of the layer is important.

for microbiological assay of Bleomycin using Mycobacterium smegmatis