For enrichment of Vibrio species from food and water samples in accordance with ISO. Vibrio is a genus of Gram negative bacteria facultative anaerobes that test positive for oxidase and do not form spores. Vibrio species is mainly responsible for causing cholera and food poisoning in humans. Vibrio cholera causes cholerae due to the intake of contaminated food. Vibrio parahaemolyticus is a major cause of food borne infections, causing food poisoning. Since Vibrio species naturally occur in sea water, there is a need for sodium chloride, although some species can grow with minimum sodium chloride concentration. The widely used media for Vibrio isolation are TCBS Agar and Alkaline Peptone Water. Alkaline Saline Peptone Water (ASPW) was first formulated by Shread, Donovan and Lee to be used as a non-selective enrichment broth for the cultivation of Aeromonas species. Alkaline Saline Peptone Water (ASPW) is in accordance with ISO/TS 21872-1:2007 which specifies a horizontal method for the detection of the two main pathogenic Vibrio species causing intestinal illness in humans: V. parahaemolyticus and V. cholera. It is applicable to products intended for human consumption and the feeding of animals, and environmental samples in the area of food production and food handling. Peptone provides amino acids and nitrogen for growth requirements, whereas sodium chloride provides essential electrolytes for maintenance of the osmotic balance. Peptone Water uses elevated pH and salt levels to provide a favorable environment for enrichment of Vibrio spa and also has an inhibitory action on the accompanying microflora.