For enrichment of Vibrio species. ALKALINE PEPTONE WATER (VEG.) is a pre-enrichment medium specially standardized for Vibrio species. Clinical samples like swabs and faeces in food and water samples can be added directly to the medium. Veg. Peptone provides nitrogenous, carbonaceous, and other important nutrients. Addition of 10 grams of seafood to 90ml of Alkaline Peptone Water and incubate it for up to 18-20 hours at 37°C. If the incubation period would get prolonged, it causes the suppressed contaminating organisms to develop. The high concentration of sodium chloride promotes the growth of Vibrio cholerae whereas the relatively high pH value of the medium suppresses the accompanying microbial flora at 18-24 hours at 35°C.