Sterile Dehydrated Culture Media

Sterile Dehydrated Culture Media ( - i r r a d i a t e d )

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Sterilization of culture media receives less attention. There are two types of sterilization processes:

  • High-pressure steam, also known as autoclaving process.
  • Sterilization using irradiation – Gamma Radiation (ionizing radiation)

Bottled media is typically sterilized by autoclaving, whereas, plate media is sterilized by irradiation.

For bottled media sterilization, the culture media is heated between 121-134°C. During this process, the nutrients of the culture media like sugar, minerals, metals, peptides may get destroyed if the heat is too high.

According to Altmann et al. and Bogokowsky, gamma radiation sterilises media in sealed plastic containers conveniently and cost-effectively, eliminating the need to sterilise the media and container separately and lowering the risk of contamination during processing.

These media can be kept for a long period in the laboratory and are always ready to use. Cobalt-60 gamma radiation is usually used as a source for sterilising. Each batch containing Gamma radiation is thoroughly measured using detectors called dosimeters, which enable parametric release

Gamma radiation is usually used for media filling in the pharmaceutical business.

Sterile dehydrated culture media are manufactured under defined production conditions and ease a range of issues in microbiology laboratories, where time, equipment, and skilled staff are in short supply.

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    Regulations

    S.No Product Name TM CODE No.
    ALTERNATIVE THIOGLYCOLLATE MEDIUM (NIH THIOGLYCOLLATE MEDIUM) (as per USP) TSM 301 See MORE
    ANTIBIOTIC ASSAY MEDIUM NO.11 (NEOMYCIN, ERYTHROMYCIN ASSAY AGAR) (as per USP) TSM 022 See MORE
    B.Q. VACCINE MEDIUM (THIOGLYCOLLATE BROTH W/ LIVER EXTRACT) TSM 216 See MORE
    E.T. MEDIUM TSM 985 See MORE
    EMB AGAR (LEVINE) TSM 371 See MORE
    FILL TEST MEDIUM TSM 2129 See MORE
    FLUID THIOGLYCOLLATE MEDIUM (as per USP) TSM 318 See MORE
    H.S. VACCINE MEDIUM (STANDARD NUTRIENT BROTH) TSM 298 See MORE
    HORSE SERUM TS014 See MORE
    LURIA BERTANI BROTH TSM 406 See MORE
    LURIA BROTH TSM 377 See MORE
    MacCONKEY AGAR W/O CV, W/0.15% BILE SALTS TSM 349 See MORE
    MacCONKEY BROTH (as per USP/EP/JP/BP) TSMH 2228 See MORE
    MacCONKEY BROTH W/ B.C.P. & NaCl TSM 529 See MORE
    NUTRIENT BROTH TSM 350 See MORE
    SABOURAUD DEXTROSE AGAR TSM 387 See MORE
    SABOURAUD DEXTROSE BROTH (as per USP/EP/JP/BP) TSMH 106 See MORE
    SOYA CASEIN DIGEST AGAR (as per USP/IP) TSMH 345 See MORE
    SOYA CASEIN DIGEST MEDIUM (TRYPTONE SOYA BROTH) (CASO BROTH) (VEG.) TSMV 332 See MORE
    SOYA CASEIN DIGEST MEDIUM WITH BCP TSM 332K See MORE
    SOYABEAN CASEIN DIGEST MEDIUM W/ MANNITOL, STERILE POWDER TSM 1938 See MORE
    SOYBEAN CASEIN DIGEST MEDIUM (as per USP/EP/JP/BP) TSMH 332 See MORE
    SOYBEAN CASEIN DIGEST MEDIUM (as per USP/EP/JP/BP) (VEG.) TSMVH 332 See MORE
    STUART TRANSPORT MEDIUM TSM 420 See MORE
    TARTOFF – HOBBS BROTH (TERRIFIC BROTH) TSM 1477 See MORE
    VIOLET RED BILE AGAR TSM 426 See MORE