Life is seldom this straightforward, and water and wastewater microbiology is no exemption.
Emerging water-borne pathogens causing bacterial diseases like cholera, typhoid fever and bacillary dysentery, etc. oblige us to have a more profound comprehension of water microbiology.

TM Media is glad to offer an extensive range of products for contemporary water and wastewater microbiology, particularly for water and wastewater engineers.

This connects TM Media indirectly with the study of water and wastewater microbiology which is incredibly rewarding: better water treatment, better wastewater treatment, safer wastewater reuse, and thus healthier individuals – in all parts of the globe.

The mandatory testing of Drinking Water made easy with TM Media view more...

Product Code
Product Name
Description
TS 004
B.P. SULPHA SUPPLEMENT

(Store below 8°C)

TS 001
EGG YOLK TELLURITE EMULSION

(Store below 8°C)

TS 002
EGG YOLK EMULSION (100 ml/vl)

(Store below 8°C)

TS 034
FRASER LISTERIA SUPPLEMENT

(Store below 8ºC)

TS 035
FRASER SELECTIVE SUPPLEMENT(LISTERIA SUPPLEMENT)

(Store below 8°C)

TS 121
LISTERIA MOXALACTAM SUPPLEMENT

(Store below 8°C)

TS 119
LISTERIA SELECTIVE SUPPLEMENT(PALCAM)

(Store below 8°C)

TS 120
OXFORD LISTERIA SUPPLEMENT

(Store below 8°C)

TS 003
POTASSIUM TELLURITE 3.5% (10 ml/vl)
TS 176
RPF SUPPLEMENT (Fibrinogen plasma trypsin inhibitor supplement)

(Fibrinogen plasma trypsin inhibitor Supplement)    (Store below 8°C)

TS 042
TTC SOLUTION 1% (10 ml/vl)

(Store below 8°C)

TS 030
UREA 40% (5 ml/vl)

(Store below 8ºC)

TM 345
Soya Bean Casein Digest Agar (TRYPTONE SOYA AGAR)

for isolation of various fastidious microorganisms with or without added blood.

(ANTIBIOTIC ASSAY MEDIUM NO. 36 )

CASEIN SOYA PEPTONE MEDIUM(as per USP/IP/EP/BP/JP)

TM 332
SOYA CASEIN DIGEST MEDIUM (TRYPTONE SOYA BROTH) CASO BROTH(ANTIBIOTIC ASSAY MEDIUM NO. 37)(ISO 9308-1:1987)

for sterility testing and cultivation of fastidious and non-fastidious microorganisms

TM 666
ASPARAGINE PROLINE BROTH

It is used for cultivation of Pseudomonas aeruginosa from water by membrane filter technique.

TM 412
AZIDE DEXTROSE BROTH

for detection of faecal Streptococci in water, sewage, food and other materials.

TM 029
AZIDE DEXTROSE BROTH W/BCP(ISO 7899-1:1984)

for cultivation of faecal Streptococci in water

TM 358
BAIRD PARKER AGAR BASE

for isolation and enumeration of coagulase positive Staphylococci from food and other products.

TM 036
BILE ESCULIN AGAR(ISO 10273:1994)

for isolation & identification of Yersinia enterocolitica from food and animal feeding products

TM 365
BRILLIANT GREEN BILE BROTH 2% (Brilliant Green Lactose Bile Broth 2%)(ISO 4831:2006 / 4832:2006)

For detection and confirmation of coliform bacteria in water and foods.    BRILLIANT GREEN BILE BROTH 2% (BRILLIANT GREEN LACTOSE BILE BROTH 2%) is used for the detection  of  coliform  organisms  in  foods,  dairy  products,  water  and  wastewater,  as  well  as  in  other materials of sanitary importance.     
Brilliant  Green  Bile  Broth  2%  contains  two  inhibitors  of  both  gram-positive  and  selected gram-negative organisms;i.e., Oxgall and Brilliant green dye.       
Peptic  digest  of  animal  tissueprovides  the  essential  nutrients  for  growth:  nitrogen,  vitamins,  minerals and  amino  acids. Organisms,  primarily  coliforms,  which  are resistant  to  the  action  of  the  inhibitors  and which ferment Lactose are able to replicate in this medium.     
Fermentation  is  detected  by  gas  production.  Gas  production  within  48  hours  is  considered  positive evidence of fermentation by coliforms. 

TM 324
BUFFERED GLUCOSE BROTH (GLUCOSE PHOSPHATE BROTH), MR-VP Medium

used in differentiation of bacteria by MR-VP test.

TM 498
CHLORAMPHENICOL YEAST GLUCOSE AGAR (as per BIS) YEAST GLUCOSE CHLORAMPHENICOL AGAR

for selective enumeration of yeasts and molds in milk and milk products. It is recommended by BIS committee under specification IS 5403:1999 Reaffirmed 2005.

TM 085
DECARBOXYLASE BROTH BASE, MOELLER (Moeller Decarboxylase Broth Base)

for differentiation of bacteria on the basis of their ability to decarboxlyate the amino acid.

TM 1802
DIFFERENTIAL REINFORCED CLOSTRIDIAL AGAR

for enumeration and cultivation of Clostridia from water.

TM 1803
DIFFERENTIAL REINFORCED CLOSTRIDIAL BROTH BASE (as per ISO 6461-1)

for cultivation of Clostridium from water.

TM 417
EC BROTH (ISO 7251:2005)

for selective enumeration of faecal and non faecal coliforms in water

TM 371
EMB AGAR, Levine (ISO 21150:2009)

for isolation, enumeration and differentiation of members of Enterobacteriaceae from pharma, dairy & food products

TM 1171
EMB AGAR, LEVINE (as per BIS)

for isolation, enumeration and differentiation of Enterobacteriaceae. It is recommended by BIS committee under specification IS: 5887 (Part I) 1976, reaffirmed 2005.

TM 294
FLUID SELENITE CYSTINE MEDIUM (Selenite Cystine Medium) (Double Pack) (ISO 6759:1993)

an enrichment medium for isolation of Salmonella species from food, dairy and clinical samples

TM 423
FLUID TETRATHIONATE MEDIUM W/O IODINE AND BG (Tetrathionate Broth Base W/O Iodine & BG)

for isolation of Salmonella from foods.

TM 1472
FRASER BROTH BASE, FRASER(ISO 11290-1:2004)

for isolation and enumeration of Listeria monocytogenes from food and animal feeds

TM 117
GN BROTH, HAJNA

for selective enrichment of gram-negative organisms of the enteric group.

TM 121
HEKTOEN ENTERIC AGAR (ISO 21567)

Hektoen Enteric Agar is used for differential & selective isolation of Salmonella and Shigella from samples.

TM 397
ISP MEDIUM NO. 2 (Yeast Malt Agar)

for cultivation of yeasts, molds and aciduric microorganisms.

TM 757
LACTOSE BROTH (Fluid Lactose Medium)

for detection of coliform bacteria in water and food products.

TM 1251
LACTOSE TTC AGAR (WITH SODIUM HEPTADECYL SULPHATE) (ISO 9308-1:2000)

for detection and enumeration of E.coli and other coliforms in water by membrane filtration technique

TM 150
LAURYL SULPHATE BROTH (Lauryl Tryptose Broth)(ISO 4831:1991)

For detection and enumeration of coliform bacteria in water, waste water, dairy products and other food sample.      LAURYL SULPHATE BROTH is usedfor the detection and enumeration of coliform bacteria in water, and food products. Lauryl  sulphate  broth  promotes  a  rich  growth  and  copious  gas  production  from small  inocula  of  coliform  organisms.  Aerobic  sporing  bacteria  are  completely  inhibited.  Tryptone provides the  nitrogen, carbon compounds, vitamins and amino acids. Lactose is the fermentable sugar. Sodium  chloride  maintains  the  osmotic  balance  of  the  medium.  Dipotassium  phosphate  and  Mono potassium  phosphate  controls  the  pH  during  fermentation  of  Lactose.  Lactose-positive  bacteria metabolize  lactose  with  gas  formation  is  indicated by  using  inverted  Durham  tubes.  Incubate  at  the wished  temperature  for  18-24  hours.  In  case  of  gas formation  the  Durham  tubes  rise  or/and  show bubbles.  Turbidity  of  the  medium  accompanied  by  formation  of  gas  within  48  hours  is  a  positive presumptive  test  for  the  presence  of E. coli and/or  other coliform organisms.  Prepared  Broth  becomes cloudy if stored at 2-8°C, but it should get cleared at room temperature. If there is fermentation in the tube  incubated  at  44°C  after  8  to  24  hours,  perform  indole  test  by  adding  Kovac‘s  reagent  (TR  008).  A positive indole test in a broth tube showing gas production at 44°C indicates the presence of Escherichia coli. If no fermentation occurs in the tube incubated at 37°C after 24 hours, the primary fermentation is assumed to be due to organisms other than coliforms.

TM 1226
LISTERIA IDENTIFICATION AGAR BASE (PALCAM)(ISO 11290-2)

for detection and enumeration of Listeria monocytogens from food and animal feeds

TM 1229
LISTERIA OXFORD MEDIUM BASE(LISTERIA AGAR BASE, OXFORD)(ISO 11290-1)

for isolation of Listeria species from pathological samples

TM 174
M-ENDO AGAR, LES (ISO 9308-1 : 2000)

for enumeration of coliforms in water using a two step membrane filter technique.

TM 781
MIU MEDIUM BASE

for detection of motility, urease and indole production.

TM 379
MacCONKEY AGAR (W/0.15% BILE SALT, CV & NaCl) (ISO 9308-2)

for selective isolation and differentiation of coliform organisms and other enteric bacteria.

TM 1036
MacCONKEY AGAR (as per BIS)

For isolation and differentiation of lactose fermenting and non-fermenting lactose enteric bacteria. It is recommended by BIS committee under specification IS: 5887 (Part I and II) 1976, reaffirmed 2005

MacCONKEY Agaris  used  forisolation  and  differentiation  of  lactose  fermenting  and  non-fermenting lactose  enteric  bacteria. The    medium    contains    Peptic    digest    of    animal      tissue    which    provides    the  nitrogeneous  and  other  essential  growth  compounds.  Sodium chloride maintains the osmotic balance of the cells.Gram-negative bacteria usually grow well on thismedia and are differentiated by their ability to ferment lactose. Lactose fermenting strains grow as red or pink and may be surroundedby a zone of acid  precipitated  bile.The  medium  turns  pink  in  case  of  lactosefermenters  andyellow  in  case  of  non-lactose-fermenters, due to neutral red when the pH of the media falls below 6.8. Lactose non-fermenting strains,  such  as Shigella and Salmonellaare  colourless  and  transparent  and  typically  donot  alter appearance of the medium.Sodium taurocholate is used as a selective agent. Agar is a solidifying agent.

TM 1238
MacCONKEY BROTH W/NEUTRAL RED (as per BIS)

For selective enrichment and enumeration of coliforms. It is recommended by BIS committee under specification IS:5887 (Part I and II) 1976, reaffirmed 2005.   MacCONKEY  BROTHW/ NEUTRAL  REDis  used  for selective  enrichment  and  enumeration  of coliforms. The  medium  contains  Peptic  digest  of  animal   tissue  which  provides  the  nitrogeneous  and    other    essential    growth    compounds.   Sodium  chloride  maintains  the  osmotic  balance  of the  cells. The selective action ofthese media is attributed to the presence of bile salts, which are inhibitory to most species  of  gram-positive  bacteria.Gram-negative  bacteria  usually  grow  well  on  these  media  and  are differentiated  by  their  ability  to  ferment  lactose.  Thecolour  change  of  the  medium  shown  by  lactose-fermenters is due to production of acid from lactose and a subsequentcolour change of the indicator dye when  the  pH  of  the  media  falls  below  6.8. Bile    salts    which    inhibits    the    growth    of    gram    positivemicroorganisms. The medium turns pink in case of lactosefermenters and yellow in case of non-lactose-fermenters,  due  to  neutral  red. Organism    producing    gas    can    be    observed    in    preparation    with  Durham  tubes. Inoculate  the  test  tubes  with  specimen, incubation  at  43 ±  1ºC  for  24-48  hours .The most probable number of coliforms per 100ml water is obtained from McGrady's tables. Confirmation of positive  results  is  carried  out  by  subculture  onto  solid  media  such  as  Endo  Agar  or  MacConkey Agar (without salt).

TM 529
MacCONKEY BROTH W/BCP & NaCl (ISO 9308-2:1990)

for presumptive identification of coliforms from water, milk and foods etc

TM 217
MILK AGAR (BROWN AND SCOTT, MODIFIED)(ISO 8360-1:1988)

for enumeration of Pseudomonas aeruginosa in swimming pool waters

TM 1038
NUTRIENT AGAR W/O NaCI (pH 6.8)

a general purpose nutrient medium for testing of water, sewage, faeces & other materials.

TM 329
NUTRIENT BROTH (W/ 1% PEPTONE) (as per IP)

sterility testing medium for aerobes and for microbial limit test.

TM 1270
NUTRIENT BROTH (W/1% PEPTONE) (as per BIS)

General purpose culture medium. It is recommended by BIS committee under specification IS: 5887 (Part II and IV) 1976, reaffirmed 2005.

TM 544
PLATE COUNT AGAR (Standard Plate Agar) STANDARD METHODS AGAR(ISO 4833-1:2013)

for determination of plate counts of microorganisms in foods, water and waste water

TM 269
R-2A AGAR

for heterotrophic plate count of treated potable water using longer incubation time.

TM 348
SIMMONS CITRATE AGAR(ISO 10273:2003)

Simmons Citrate Agar for differentiation of Enterobacteriaceae on the basis of citrate utilization

TM 440
TERGITOL - 7 AGAR BASE (as per BIS)

for selective isolation and identification of coliform bacteria from water. It is recommended by BIS committee under specification IS: 5887 (Part I) 1976, reaffirmed 2005

TM 424
TRIPLE SUGAR IRON AGAR

for confirmation of gram negative enteric bacilli on basis of dextrose, lactose and sucrose fermentation and H2production.

TM 899
TRYPTOPHAN MEDIUM (TRYPTOPHAN BROTH)

for detection of indole production.

TM 1314
VIOLET-RED BILE AGAR (as per BIS)

For selective isolation, detection and enumeration of coli-aerogenes in water, milk products. It is recommended by BIS committee under specification IS 5401 (Part 1):2002.

VIOLET RED BILE AGAR is used for the isolation and confirmation of coli - aerogenes in water, milk and other dairy products. Peptone and Yeast extract are a source of nitrogen, sulphur, carbon, vitamins and minerals. Bile salts and crystal violet are the inhibitors of gram-positive microorganisms. Lactose is the fermentable carbohydrate. Neutral red change to red-purple due to the building of acid during fermentation which change the pH. Sodium chloride is for the osmotic balance. It relies on the use of the selective inhibitory components crystals violet and bile salts and the indicator system lactose, and neutral red. Other gram-negative bacteria can be suppressed by incubation at temperature over 42°C for 18 hours or anaerobic incubation. Agar is the solidifying agent. Lactose fermenting coliforms give red colonies with precipitation of bile salts. Lactose non-fermenters and late lactose fermenters produce pale colonies.

 

The Enterobacteriaceae group includes lactose-fermenting coliforms bacteria and non-lactose fermenting species like Salmonella and Shigella.

TM 492
XLD AGAR(ISO 6579:2002)

XLD Agar is used for selective isolation and enumeration of Salmonella typhi and other Salmonella species. 

XLD  Agar  was  formulated  by  Taylor  for  the  isolation  and  differentiation  of  enteric  pathogens including Salmonella  Typhi from other  Salmonella species. XLD Agar has been recommended for the identification  of Enterobacteriaceae  and  for  the  microbiological  testing  of  foods,  water  and  dairy products. The media formulation does not allow the over growth of other organisms over Salmonella and Shigella.   

The  medium  contains  Yeast  extracts  as  source  of  vitamins  and  minerals.  Addition  of  Sodium deoxycholate acts as a selective agent which is inhibitory to Gram-positive bacteria. It suppresses the growth of other enteric pathogens and enhances the growth of only few enteric bacilli. The medium contains Xylose as a fermentable carbohydrate which is utilized by Salmonella species. The medium pH is changed due to fermentation of Xylose which is detected by indicator Phenol red, thus the colony colour turns red. The medium also contains Lactose and Sucrose as the source of fermentable sugar.  L-lysine  is  an  essential  amino  acid  source.  Lysine  is  added  to  differentiate Salmonella  Sp.  
Sodium  chloride  helps  maintaining  the  osmotic  balance  of  the  cells.  This  medium  also  allows differentiation of bacilli based on their ability to produce H2S; it contains Sodium thiosulphate and Ferric  ammonium  citrate  that  helps  visualizing  the  black  centered  colonies  on  production  of hydrogen sulphide in the medium. Agar is added as the solidifying agent.

TM 1826
TSC AGAR BASE (PERFRINGENS AGAR BASE) (ISO 7937: 2004, ISO 14189:2013

For enumeration of Clostridium perfringens from food. TSC AGAR BASE (As per ISO) is used for detection and enumeration of Clostridium perfringens. Medium consists of Tryptose, Peptic digest of soyabean meal and Yeast extract as source of nitrogenous compounds, carbon, sulphur, vitamin B complex and trace elements essential for Clostridial growth. Sodium metabisulphite and Ferric ammonium citrate act as an indicator of sulphite reduction, indicated by black coloured colonies. S.F.P supplement (TS 0054) or T.S.C supplement (TS 076) help in the selective isolation of C. Perfringens by inhibiting accompanying flora. These antibiotics can also be added to the sterile, liquefied culture medium in the form of filter-sterilized solutions. Egg yolk emulsion serves as a source of lecithin utilized by C. Perfringens. No lecithinase activity can be detected without egg yolk but not all Clostridium perfringens strains show an opaque zone after overnight incubation. So both black lecithinase-positive and black lecithinase-negative colonies should be considered as presumptive Clostridium perfringens on TSC Agar Base and need further confirmatory tests like nitrate reduction, lactose fermentation, gelatin liquefaction and the absence of motility.

TM 1827
YEAST EXTRACT AGAR (ISO 6222:1999)

For enumeration of microorganisms from water. YEAST EXTRACT AGAR (as per ISO) is used for plate count of microorganisms in water. Yeast Extract and Tryptone and Yeast extract provide sources of nitrogen, amino acids, essential vitamins, and carbon required for organism growth. Agar is the solidifying agent. In nature, all water contains some impurities. As we know that microbial pathogens are often found in water frequently as a result of fecal matter from sewage discharges, leaking septic tanks, or runoff from animal feedlots. As water flows in streams, sits in lakes, and filters through layers of soil and rock in the ground, it dissolves or absorbs the substances that it touches. The combination of media ingredients and the appropriate incubation temperature of 35°C and 22°C allow the detection of a large number of microbial contaminants. Inoculate the culture medium by the pour-plate method or by spreading the material on the surface. Incubation period can extend up to 7 days also at 28 °C aerobically.

TM 1038A
NUTRIENT AGAR (pH 6.8) (ISO 10273-2003-06)

A general purpose nutrient medium for testing of water, sewage, faeces & other materials. NUTRIENT AGAR (pH 6.8) is used as a general purpose medium for testing of water, sewage, faeces & other materials. It is a basic culture medium used for maintenance or to check purity of subcultures prior to biochemical or serological tests. This medium is recommended for presumptive isolation of Salmonella and Yersinia colonies. Nutrient Agar, pH 6.8 has relatively simple formulation which provides the necessary nutrients for the growth of many microorganisms which are not very fastidious. Many bacteria have the optimum pH growth range of 6.6 to 7.0.