The Pharmaceutical Microbiological assessment utilizes an assortment of culture media relying on the application required.
TM Media is glad to presents Pharmaceutical Microbiological Culture media that is basically significant for the majority of microbiological examination. Drug efficacy & safety are the foremost concern of Pharmaceutical Industry. Pathogenic bacteria, fungi (yeasts and moulds) and toxins produced by microorganisms are all likely contaminants of medicines- albeit stringent, regulated processes are in place to ensure the risk is minimal and this is only attainable with high quality media then the prospect of achieving accurate, reproducible and repeatable microbiological test results are high.
TM Media also involved in the production of full range of culture media for sterility test & non-sterile microbial examination of pharmaceutical products, according to Harmonized Microbial Limit Test (USP, JP, EP, BP).
Whenever you require ensuring sterility, examine antimicrobial effectiveness, microbial contamination or bioburden, evaluating endo-toxins or recognizing microbes from environmental monitoring processes, we are here to help.
We will work with you as part of your team to offer broad quality products as well as convenient and compelling solution for any difficulties that you confront.

Product Code
Product Name
Description
TS 002
EGG YOLK EMULSION (100 ml/vl)

(Store below 8°C)

TS 217
GENTAMYCIN SUPPLEMENT

(Store below 8°C)

TS 005
POTASSIUM TELLURITE 1% (1 ml/vl)

(Store below 8°C)

TM 1729
ANTIBIOTIC ASSAY MEDIUM NO.1 (as per USP)

for microbiological assay of β-lactam & other antibiotics in pharmaceutical and food rated products.

TM 1731
ANTIBIOTIC ASSAY MEDIUM NO. 2 (as per USP)

for microbiological assay of antibiotics.

TM 1734
ANTIBIOTIC ASSAY MEDIUM NO. 3 (as per USP)

for turbidimetric or serial dilution assay of various antibiotics.

TM 1743
ANTIBIOTIC ASSAY MEDIUM NO. 8 (BASE AGAR W/ LOW pH) (as per USP)

for use in the plate assay of tetracycline and other antibiotics

TM 1751
ANTIBIOTIC ASSAY MEDIUM NO. 11 (as per USP)

for microbiological assay of antibiotics.

TM 345
Soya Bean Casein Digest Agar (TRYPTONE SOYA AGAR)

for isolation of various fastidious microorganisms with or without added blood.

(ANTIBIOTIC ASSAY MEDIUM NO. 36 )

CASEIN SOYA PEPTONE MEDIUM(as per USP/IP/EP/BP/JP)

TM 946
BISMUTH SULPHITE AGAR (as per USP)

for selective isolation of Salmonellae from faeces, urine, sewage and other meterials.

TM 951
BRILLIANT GREEN AGAR, MODIFIED (Brilliant Green Agar Medium)as per USP

for isolation of Salmonellae other than Salmonella typhi from faeces, foods and dairy products.

TMH 101
BUFFERED NaCl - PEPTONE SOLUTION (as per USP/EP/JP/BP)

dilution fluid for samples in case of microbiological contamination.

TM 1665
CETRIMIDE AGAR MEDIUM (as per USP)

for selective isolation of Pseudomonas aeruginosa.

TMH 113
CETRIMIDE AGAR (as per USP/EP/JP/BP)

for the selective isolation of gram-negative bacteria like, Pseudomonas aeruginosa.

TMH 116
COLUMBIA AGAR (as per USP/EP/JP/BP)

For selective and nutritive for Clostridium species.     COLUMBIA   AGAR is  a  highly  nutritive  and  selective  medium  for  the cultivation  of  fastidious organisms, especially when used as a base for blood chocolate agar. It can also be used as a selectiveisolation medium by adding antimicrobial agents. It is a nutritiously rich general purpose medium developed  by  Ellner  et  al5  from  Columbia  University.  Columbia  Agar  conforms  to  harmonized USP/EU/JP requirements.1,2,3,4 Nitrogen, vitamins, and amino acids are provided by a combination of Pancreatic digest of Casein, Peptic digest of Meat and Pancreatic digest of Heart. Maize starch is included to supply a carbon source. Yeast extract provides B-complex vitamins. Sodium chloride maintains the osmotic balance of the medium. Agar acts as a solidifying agent. Supplementation with blood (5 - 10%) provides additional growth factors for fastidious microorganisms, and aids in determining hemolytic reactions. Hemolytic patterns may vary with the source of animal blood and the type of basal medium used.6 In general, blood agar bases are relatively free of reducing sugars, which have been reported to adversely influence the hemolytic reactions of hemolytic streptococci. Inoculate Columbia Agar (TMH 116) medium with a small number (not more than 100 cfu) of the appropriate microorganism and incubate under anaerobic conditions at 30 - 35°C for 18 - 48 hours. For isolation purpose, streak withinoculating loop on Columbia Agar (TMH 116) and incubate under anaerobic conditions at 30-35ºC for 48 hours.       
For Columbia Agar, supplemented with blood, examine the hemolytic reactions after 18 - 24 and 48 hoursincubation. There are four types of hemolysis on blood agar media described as7:     
1. Alpha hemolysis (α) is the reduction of hemoglobin to methemoglobin in the medium surrounding the colony. This produces a green discoloration of the medium.     2. Beta hemolysis (β) is the lysis of red blood cells, producing a clear zone surrounding the colony.      
3. Gamma hemolysis (γ) indicates no hemolysis. No destruction of red blood cells occurs and there is no change in the medium.      
4. Alpha-prime-hemolysis (α’) is a small zone of complete hemolysis that is surrounded by an area of partial lysis.   

TM 1526
COLUMBIA AGAR (as per USP)

For detection of Clostridium perfrigens from pharma products.      COLUMBIA  AGAR is  a  highly  nutritive  and  selective  medium  for  the cultivation  of  fastidious organisms, especially when used as a base for blood chocolate agar. It can also be used as a selectiveisolation  medium  by  adding  antimicrobial  agents.  It  is  a  nutritiously  rich  general  purpose  medium developed  by  Ellner  et  al5  from  Columbia  University.  Columbia  Agar  conforms  to  harmonized USP/EU/JP requirements.1,2,3,4  Nitrogen, vitamins, and  amino acids  are  provided  by a combination of Pancreatic digest of Casein, Peptic digest of Meat and Pancreatic digest of Heart. Maize starch is included to supply a carbon  source. Yeast extract provides  B-complex vitamins. Sodium chloride maintains the osmotic balance of the medium. Agar acts as a solidifying agent. Supplementation with blood (5 - 10%) provides  additional  growth  factors  for  fastidious  microorganisms,  and  aids  in  determining  hemolytic reactions. Hemolytic patterns may vary with the source of animal blood and the type of basal medium used.6 In general, blood agar bases are relatively free of reducing sugars, which have been reported to adversely influence the hemolytic reactions of hemolytic streptococci. Inoculate Columbia Agar medium with  a  small  number  (not  more  than  100cfu)  of  the  appropriate  microorganism  and  incubate  under anaerobic conditions at 30 - 35°C for 18 - 48 hours. For isolation purpose, streak with inoculating loop on Columbia Agar and incubate under anaerobic conditions at 30-35ºC for 48 hours.      
For Columbia Agar, supplemented with blood, examine the hemolytic reactions after 18 - 24 and 48 hoursincubation. There are four types of hemolysis on blood agar media described as7:       
1. Alpha hemolysis (α) is the reduction of hemoglobin to methemoglobin in the medium surrounding the colony. This produces a green discoloration of the medium.     2. Beta hemolysis (β) is the lysis of red blood cells, producing a clear zone surrounding the colony.         
3. Gamma hemolysis (γ) indicates no hemolysis. No destruction of red blood cells occurs and there is no change in the medium.       
4. Alpha-prime-hemolysis (α’) is a small zone of complete hemolysis that is surrounded by an area of partial lysis.    

TM 447
DIAGNOSTIC THIOGLYCOLLATE MEDIUM W/O INDICATOR (THIOGLYCOLLATE MEDIUM W/O INDICATOR)

for enrichment of blood cultures.

TMH 107
EE BROTH MOSSEL (ENTEROBACTERIA ENRICHMENT BROTH, MOSSEL) (as per USP/BP/JP/EP)

for selective enrichment of Enterobacteriaceae.

TM 1537
FLUID LACTOSE MEDIUM (Lactose Broth) (as per USP)

for detection of coliform bacteria in water, foods and dairy products.

TM 318
FLUID THIOGLYCOLLATE MEDIUM (THIOGLYCOLLATE MEDIUM, FLUID) (as per USP/EP/BP/JP)

For sterility testing of biologicals and for cultivation of aerobic, anaerobic and microaerophilic organisms.       FLUID  THIOGLYCOLLATEMEDIUMis  usedfor  sterility  testing  of  biological  and  for  cultivation  of anaerobic,  and  microaerophilic  organisms.“Brewer”  formulated  this  mediumand  is  recommended  by USP.The  mediais  also  usedfor  sterility  testing  of  antibiotics, biologicaland  foods  andalso for determining  the  phenol  coefficient  and  sporicidal  effect  of  disinfectants.  However,  it  is  intended  for  the examination of clear liquid or water-soluble materials. Fluid Thioglycollate Medium is also routinely used to check the sterility of stored blood in blood banks.The mediumcontains Pancreaticdigest of casein andYeast   extract thoseprovidesthe nitrogen   and   essential growth enhancersnecessary   for   bacterial multiplication.  Sodium  thioglycollate and L-Cystineactsas  a  reducing  agent which  maintains    a    low oxygen tension  by  removing  molecular  oxygen  from  the  environment.Indicator Resazurin dispensed throughout  the  media  causing  the  media  to  appear  pink  in  colourin oxidizedstate.Dextrose  is  a fermentable energy source. Addition of small amount of agar helps in maintaining low redox potential for stabilizing  the  medium. Addition  of  agar  in  small amount favors  the growth  of  aerobes  as  well  as anaerobeswhich retards dispersion of CO2, diffusion of oxygen, and reducing substances.On incubation at30 -350C  for  48-72  hours  shown  luxuriant  growth  in  all  the  culture  strains  tested.Sodium  chloride helps maintaining the osmotic balance.

TMH 110
MacCONKEY AGAR (as per USP/EP/BP/JP)

For the isolation, enumeration and enrichment of Enterobacteriaceae.

MacCONKEY AGAR is  used  for  detection  and  isolation  of  Gram-negative  organisms  fromwater4,pharmaceutical1,2,3and  industrial5sources.  MacCONKEY AGAR  conforms  to  harmonized  USP/EU/JP1,2,3. Pancreatic   digest   of   Gelatin   is   the nitrogen   and   vitamin   sources   in   MacConkey Agar.   Lactose Monohydrate  is  the  fermentable  carbohydratewith  Neutral  red  serving  as  the  pH  indicator.Sodium chloride maintains the osmotic balance. Agar is the solidifying agent. Bile salts mixture and Crystal violet are  the  selective  agents,  inhibiting  Gram-positive cocci  and  allowing  Gram-negative organisms to  grow. Lactose fermenting strains grow as red or pink and may be surrounded by a zone of acid precipitated bile. The red colour is due to production of acid from lactose, absorption of Neutral red and a subsequent colour change of  the  dye  when  the  pH  of  medium  falls  below  6.8.  Lactose  non-fermenting  strains,  such  as Shigella and Salmonella are  colourless  and  transparent  and  typically  do  not  alter  appearanceof  the  medium. Inoculate MacCONKEY AGAR  (TMH  110) medium  with  a  small  number  (not  more  than  100  cfu)  of  the  appropriate microorganism  and  incubate  at35±2°C  for  18-24  hours.  The  growth  of  colonies  indicates  the  possible presence of Escherichia coliand it shows red colour colonies.

TMH 109
MacCONKEY BROTH

For the detection enumeration of coliform bacteria.     MacCONKEY  BROTH  is  used for  the  selective  enrichment  and  enumeration  of  coliform  bacteria.  It conforms to harmonized USP/EP/JP requirements.1,2,3 MacConkey Broth is a modification of the original Bile  salt  broth  recommended  by  MacConkey,  containing  0.5%  sodium  taurocholate  and  litmus  as  an indicator.4MacConkey suggested further variations of this formula using neutral red indicator instead of litmus.5,6Childs  and  Allen  demonstrated  the  inhibitory  effect  of  neutral  red  and  substituted  the  less inhibitory Bromcresol purple.7 Pancreatic digest of Gelatin provides necessary nitrogen source. Lactose is the  carbohydrate  for  Gram-negative  lactose-fermenting  bacilli.  Sodium  chloride  maintains  the  osmotic balance of the cells. Dehydrated Ox bile inhibit the growth of Gram-positive organisms. Gram-negative bacteria  usually  grow  well  on  this  media  and  are  differentiated  by  their  ability  to  ferment  lactose. Lactose-fermenting  organisms  grow  very  well  in  MacConkey  Broth  and  produce  acid,  causing  the medium  to  turn  yellow.  Gas  is  also  produced,  which collects  in  the  Durham  tubes.  Non-fermenting organisms  produce  good  growth  but  will  not  produce acid  or  gas.  Inoculate  10ml.  MacConkey  Broth (TMH 109) medium with a small number (not more than 100 cfu) of the appropriate microorganism and incubate at 35±2°C for 18-24 hours. Subculture on a plate of MacConkey Agar (TMH 110) and incubate at 35±2°C for 18-24 hours.

TMH 114
MANNITOL SALT AGAR BASE (as per USP/BP/EP/JP)

For the selective isolation and enumeration of Staphylococci.    MANNITOL SALT AGAR is used for the selective isolation and enumeration of Staphylococci species. It conforms  to  harmonized  USP/EP/JP  requirements.1,2,3  Chapman  formulated  Mannitol  Salt  Agar  to isolate staphylococci by inhibiting growth of most other bacteria with a high salt concentration.4Mannitol is  the  fermentable  carbohydrate  which  leads  to  acid  production,  detected  by  Phenol  red  indicator. Staphylococcus aureus ferment  mannitol  and  produce  yellow  coloured  colonies  surrounded  by  yellow zones.  The  lipase  activity  can  be  visualized  as  yellow  opaque  zones  around  the  colonies.5  Coagulase-negative strains of Staphylococcus aureus are usually mannitol non-fermenters and therefore produce pink to  red  colonies  surrounded  by  red-purple  zones.  Presumptive  coagulase-positive  yellow  colonies  of Staphylococcus  aureus   should   be   confirmed   by   performing   the   coagulase   test.   Lipase   activity   of Staphylococcus aureus can  be  detected  by  supplementing  the  medium  with  egg  yolk  emulsion.  Sodium chloride in high concentration serves as an inhibitory agent against bacteria other than Staphylococci. Beef extract  gives  essential  growth  factors  and  trace  nutrients  to  the  growing  bacteria.  Phenol  Red  is  the pH indicator.  Agar  acts  as  the  solidifying  agent.  Inoculate  Mannitol  Salt  Agar  (TMH  114)  medium  with  a small number (not more than 100 cfu) of the appropriate microorganism and incubate at 35 - 37°C for 18 - 48  hours.  Recovery  rate  is  considered  as  100%  by  sub  culturing  on  Soyabean  Casein  Digest  Agar  (TMH 103) at 35±2°C for 18 - 48 hours.

TMH 105
POTATO DEXTROSE AGAR

For isolation and selection of fungi. 

POTATO DEXTROSE AGAR is used for plate counts of yeasts and molds in the examination of Pharmaceutical products. It conforms to harmonized USP/BP/EP/JP requirements for the growth of fungi.1,2,3,4 Potato Dextrose Agar can be used for growing clinically significant yeast and molds.5 Adjusting the pH of the medium by tartaric acid to 3.5 inhibits the bacterial growth. Heating the medium after acidification should be avoided as it may hydrolyze the agar which can render the agar unable to solidify. The nutritionally rich base Potato infusions encourage specially yeast and mold growth. Dextrose is the fermentable carbohydrate as carbon and energy source. Agar is the solidifying agent. Inoculate Potato Dextrose Agar (TMH 105) medium with a small number (not more than 100 cfu) of the appropriate microorganism and incubate at 20-25°C for 2-5 days.

TM 1279
PSEUDOMONAS AGAR F (FOR FLUORESCEIN) (as per USP)

for detection of fluorescein production by Pseudomonas species.

TM 1280
PSEUDOMONAS AGAR P (FOR PYOCYANIN) (as per USP)

for detection of pyocyanin production by Pseudomonas species.

TMH 111
RAPPAPORT VASSILIADIS SALMONELLA ENRICHMENT BROTH

for selective enrichment & isolation broth for Salmonella species. RAPPAPORT   VASSILIADISSALMONELLA   ENRICHMENT   BROTH is   used   for   enrichment   and selective   isolation   of Salmonella species.   It   conforms   to   harmonized   USP/EP/JP   requirements.1,2,3Rappaport et  al.4formulated  an  enrichment  medium  for Salmonella species.that  was  modified  by Vassiliadis et  al.5Rappaport  Vassiliadis  Salmonella  Enrichment  Broth  medium  is  evaluated  as  an alternative  of  Rappaport-Vassiliadis  (RV)  Broth  where  Soya  Peptone  has  replaced  Enzymatic  Digest  of Casein  as  the  nitrogen  and  vitamin  sourcewhich  has been  reported  to  enhance  the  growth  of salmonellaspecies.6,7This  medium  is  veryhygroscopic  and  must  be  protected  from  moisture. Sodium chloride  maintains  the osmotic  balance  in  the  medium.  The  low  pH  of  the  medium,  combined  with  the  presence  of  Malachite green and Magnesium chloride raises the osmotic pressure and Potassium dihydrogen phosphate acts as a  buffer,  selective  for  the  highly  resistant Salmonella species.  Malachite  green  is  inhibitory  to  organisms other  than Salmonella sp.  Novobiocin  is  added  as  a  selective  agent. When  Rappaport  Vassiliadis Salmonella Enrichment Broth medium is combined with direct culture and Selenite enrichment, 98.9% of Salmonellaare recovered

TM 1596
RAPPAPORT VASSILIADIS SALMONELLA ENRICHMENT BROTH (as per USP)

For selective enrichment of Salmonella species under high osmotic conditions and low pH.    RAPPAPORT VASSILIADIS SALMONELLA ENRICHMENT BROTH used for selective enrichment of Salmonellae. The composition of the medium was developed by “Rappaport” following the observation that Salmonella   was   more   resistant   to   hypertonic   media   than   most   other enterobacteria.   In   his experiments,  Rappaport  showed  that  magnesium  chloride  was  the  most  effective  of  all  the  salts  tested. Finally,  van  Schothorst  and  Renaud  modified  RappaportVassiliadis  Broth  by  replacing  the  Casein peptone  with  a  Soyapeptoneas  the carbon  and  nitrogen  sources  for  general  growth  requirements.  By incorporating  a Sodium  chloride,Potassium  phosphate,  monobasic  and  Dipotassium phosphate  buffer into the formula, resulting in a greater stability of the medium over time.Selectivity of the medium was increased  still  further  by  the  addition  of  malachite  green.  Malachite  Green  is  inhibitory  to  organisms other  than Salmonella spp.  The  low  pH  of  the  medium,  combined  with  the  presence  of Malachite  Green and Magnesium Chloride, select for the highly resistant Salmonellaspp.      

TMH 115
REINFORCED CLOSTRIDIAL BROTH (as per USP/BP/JP/EP)

for isolation, cultivation and enumeration of Clostridium species, highly nutritive for Clostridium sporgenes and other anaerobes.

TMH 104
SABOURAUD DEXTROSE AGAR

For selection, isolation and cultivation of yeasts and fungi.   SABOURAUD  DEXTROSE  AGAR isused  for  the  cultivation  dermatophytes,  other pathogenic  and nonpathogenic  fungi  and  yeasts.  It  conforms  to  harmonized  USP/EP/JP  requirements.1,2,3Sabouraud Dextrose Agar is a modification of Dextrose Agar described by Sabouraud.4 Mixture of Peptic digest of animal tissue & Pancreatic digest of Casein provides carbon and nitrogen required for the growth of a wide  variety  of  organisms.  The  high  Dextrose  concentration  and  acidic  pH  of  the  formula  permits selectivity  of  fungi.5  Agar  is  incorporated  into  the  media  as  a  solidifying  agent.  George  enhanced Sabouraud Dextrose Agar with the addition of cycloheximide, streptomycin, and penicillin to produce anexcellent medium for the primary isolation of dermatophytes.6 The medium is often used with antibiotics for the isolation of pathogenic fungi from material containing large numbers of other fungi and bacteria. The fungi maintain their typical cultural appearance and thus may be readily identified according to the standard macroscopic characters described by Sabouraud. Aseptically add 0.5gms Cycloheximide, 40,000 units Streptomycin, 20,000 units Penicillin and to each litre of autoclaved, cooled medium. Alternatively, one  may  add  0.4gms  chloramphenicol  and  0.05gms  cycloheximidS  to  each  liter  of  medium  before autoclaving. The medium is usually inhibitory to most non-pathogenic fungi and bacteria by the addition of antibiotics as above. Inoculate Sabouraud Dextrose Agar (TMH 104) medium with a small number (not more than 100 cfu) of the appropriate microorganism and incubate at 20 - 25°C for 48 - 72 hours.

TMH 106
SABOURAUD DEXTROSE BROTH

for for selection, isolation & cultivation of yeasts, molds and aciduric microorganisms.

TMH 103
SOYABEAN CASEIN DIGEST MEDIUM (TRYPTONE SOYA BROTH) (CASO AGAR) (as per USP/EP/JP/BP)

for the cultivation of microorganisms sterility testing of molds and bacteria.

TMH 102
SOYA CASEIN DIGEST MEDIUM (TRYPTONE SOYA BROTH) (CASO BROTH) (as per USP/EP/JP/BP)

for the cultivation of microorganisms sterility testing of molds and bacteria.

TMH 108
VIOLET RED BILE DEXTROSE AGAR (as per USP/BP/EP/JP)

for the detection and enumeration of coliform bacteria.

TM 1627
VIOLET RED BILE AGAR W/GLUCOSE & LACTOSE (as per USP)

For selective isolation, detection and enumeration of coli aerogenes gram negative bacteria. 

Dissolve 50.63gms in 1000ml distilled water. Gently heat to boiling with gentle swirling and dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 45ºC and immediately pour into sterile Petri plates.

Appearance: Reddish-purple, clear to slightly opalescent -:

pH (at 25ºC): 7.4 ± 0.2

 

TM 1722
VOGEL JOHNSON AGAR MEDIUM (as per USP)

for selective isolation of coagulase positive, mannitol fermenting S.aureus from foods & clinical samples.

TM 1790
XLD AGAR (as per USP)

For isolation and identification of Salmonella typhi and other Salmonella species.

XLD AGAR or (XYLOSE LYSINE DEOXYCHOLATE AGAR)(as per USP)was formulated by Taylor, it is a differential media used for isolation and differentiation of enteric pathogens particularly Shigellaand Salmonella. The medium contains Yeast extracts as source of vitamins and minerals. Addition of Sodium desoxycholate  acts  as  a  selective  agent  which  is  inhibitory  to  Gram-positive  bacteria.  It  suppresses  the growth  of  other  enteric  pathogens  and  enhances  the  growth  of  only  few  enteric  bacilli.    The  medium contains Xylose as a fermentable carbohydrate which is utilized by Salmonellaspecies.  The medium pH is changed due to fermentation of Xylose which is detected by indicator Phenol red, thus the colony colour turns red. The medium also contains Lactose and Sucrose as the source of fermentable sugar. L-lysine is an  essential  amino  acid  source.  Lysine  is  added  to  differentiate Salmonella sp.  Sodium  chloride  helps maintaining  the  osmotic  balance  of  the  cells. This medium  also  allows  differentiation  of  bacilli  based  on their  ability  to  produce  H2S,  it  contains  Sodium  thiosulphate  and  Ferric  ammonium  citrate  that  helps visualizing  the  black  centered  colonies  on  production  of  hydrogen  sulphide  in  the  medium.  Agar  is added as the solidifying agent.

TMH 112
XLD AGAR (Xylose Lysine Deoxycholate Agar) (as per USP/BP/JP/EP)

for selective differentiation and enrichment medium for Salmonella and Shigella species.

TM 1739
ANTIBIOTIC ASSAY MEDIUM NO. 5

for use in the potency assay of streptomycin