Anaerobe Indicator Tablet is used for the detection of anaerobic conditions in the jar. It is a sachet containing a tablet prepared from resazurin. It is used for checking the anaerobic atmosphere in sealed containers used for incubation. The colour change from purplish blue to pink indicates anaerobic conditions.
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The Anaerobe Indicator Tablet is used to verify that anaerobic (oxygen-free) conditions exist inside a closed incubation jar. It is used as a visual indicator to verify that the environment is correct for anaerobic microorganisms.
The resazurin dye in the tablet is colour-dependent on the level of oxygen. In the presence of oxygen, it turns purplish-blue. As anaerobic conditions are created, the tablet will turn pink, showing a healthy anaerobic environment.
Just peel off the tablet from its covering, leaving the transparent cover undisturbed. Put it along with the included pouch inside the Anaerobic Jar, and then seal the jar for incubation. The tablet is good enough for up to 3.5 liter capacity jars. It might take a few hours for the complete colour change to pink.
If the tablet is still purplish-blue, it means anaerobic conditions have not been attained in the jar. This may be because of a leak in the seal, a problem with the anaerobic gas generation system (if used), or a lack of time for the anaerobic environment to set up.
No, the Anaerobe Indicator Tablet is single-use only. After it has been used to show anaerobic conditions, it must be discarded. Using a new tablet for every incubation guarantees accurate results.
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