HALOPHILIC BROTH

Halophilic media are formulated for isolation and cultivation of extreme halophilic species of Halobacterium and Halococcus from foods. For optimum growth they require high salt concentration of about 20-30%. In general, the requirement for salt by halophilic microorganisms is not an exclusive need for NaCl since many species require low levels of K +, Mg++ and other ions in addition to NaCl. The level of salt required by microorganism varies greatly. Therefore, the microbial types associated with a particular salted food depend on the concentration and type of salt and food. The most recent classifications of halophilic microorganisms are based on the level of salt required. These bacteria can cause pink discoloration on the outer surface accompanied by putrefaction and decomposition of fish, bacon and hides preserved in sea salts.

for isolation and cultivation of halophilic bacteria